Just in time for SCP2019, we have a new preprint led by Harrison Specht released on bioRxiv titled ‘High-throughput single-cell proteomics reveals the emergence of macrophage heterogeneity’. In this manuscript we describe the various sample processing and computational advances that have come together to form our next-generation method for single-cell proteomics: SCoPE2. We were able to apply this method to the study of monocyte cells differentiating to macrophages. We showed that the macrophages had increased heterogeneity over the monocytes, and that this heterogeneity included reciprocal expression of genes known to be unregulated in M1 or M2-polarised macrophages. The paper can be read here.