Led by Frazer, this was a fun side project for the lab, including our three MRes project students in 2025 – Markella, Charlotte and Shu. In it, they showed that current T7 based MNV reverse genetics can be performed without IVT or helper virus by co-transfecting plasmmids encoding T7 RNA polymerase and vaccinia capping enzymes. They showed further boosts in the efficiency of this system by expressing the CD300lf receptor for mouse norovirus on the non-permissive BSR-T7 cells typically used for this work. You can read about this work on bioRxiv here.
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