Dr Ed (Edward) Emmott (PI)

Background: Ed joined the Centre for Proteome Research/Department of Biochemistry at the University of Liverpool to start the Emmott lab as a Tenure-Track Fellow in November 2019.

Research Interests: +ssRNA virus replication, virus-host interactions especially viral proteases & viral modification of ribonucleoprotein complexes (e.g. ribosomes), ultrasensitive/single-cell proteomic methods.

For more on Ed, click here.

Ed moved to the University of Liverpool in November 2019 to set up the lab  as part of the Centre for Proteome Research, Department of Biochemistry. The labs goal is the use and development of proteomic methods to study virus replication and virus-host interactions.

Previously, he was a Postdoc in Nikolai Slavov’s lab, part of the Department of Bioengineering and the Barnett Institute for Chemical and Biological Analysis at Northeastern University. His postdoctoral research with Nikolai involved the study of ribosome heterogeneity and the immune response, as well as the development and application of single cell proteomic methods (SCoPE-MS/SCoPE2).

Prior to joining Nikolai’s lab, Ed was a postdoc in Ian Goodfellow’s lab in the Department of Pathology at the University of Cambridge and Imperial College London where he studied norovirus replication and virus-host interactions, becoming particularly interested in the role of the viral protease, and polyprotein processing.

His PhD was at the University of Leeds  under the supervision of Julian Hiscox with his thesis on ‘High-throughput proteomic analysis of the interactions of avian coronavirus and its nucleocapsid with the host cell’. His Bachelors was from the University of Warwick in Medical Microbiology & Virology where his undergraduate research on Adenovirus interactions with PML bodies was supervised by Keith Leppard.

Twitter: @edemmott  ORCID: 0000-0002-3239-8178   CPR Page: (CPR)   Staff Page: (Liverpool)

Email: e.emmott@liverpool.ac.uk

 

Stephen Holmes (PhD Student)

Background: Stephen joined the Emmott lab to start his PhD on proteolytic cleavage during virus infection and single-cell proteomics in October 2021.

Research Interests: Viral proteases, SARS-CoV-2, norovirus, SCoPE2.

For more on Stephen, click here.

I graduated from the University of Liverpool in 2019 with a BSc in Tropical Disease Biology. My module choices for this degree were focused towards virology and molecular biology. My undergraduate dissertation explored the role of a protein called Myeloid Nervy, and DEAF1 (MYND) in trypanosomes, the causative agent of African sleeping sickness. While carrying out this project I developed a strong interest in the roles of proteins during infection. Following my undergraduate degree, I completed an MSc degree in Tropical Disease Biology in the Liverpool School of Tropical Medicine where again, I studied the molecular biology of parasites, viruses and bacteria. With a heavier focus on virology.  My dissertation project was a bioinformatics project focused on exploring the role of Diflavin proteins in Tsetse fly species. Here I focused on three proteins in particular Nitric oxide synthase, NADPH-dependent diflavin oxidoreductase 1 and Cytochrome P450 reductase. After my graduation, I spent 9 months working as an associate practitioner in virology for the NHS. Here, my primary role was to screen frontline staff for SARS-CoV-2 using Loop-Mediated Isothermal Amplification (LAMP).

I am currently carrying out my PhD entitled  “Mass-spectrometry-based proteomic analysis of protease activity during SARS-CoV-2 infection.”, which will be supervised by Dr. Edward Emmott and Prof. Julian Hiscox. The project will be looking at exploring the viral and host cell proteome for proteolytic cleavage events during SARS-CoV-2 infection using  mass spectrometry-based proteomics and SCoPE2.

 

 

 

Sophie Morris (MRes Student)

Background: Sophie joined the Emmott lab to start her MRes project on norovirus post-translational modifications in September 2021.

Research Interests: Norovirus, Post-translational modifications.

For more on Sophie, click here.

I graduated from University of Leicester in 2021 with a BSc in Medical Microbiology. During my undergraduate studies I undertook a placement year at Micropathology Ltd. During my placement year, alongside diagnostic duties, I carried out a research project investigating the distribution of HHV-6B infection in febrile children, this involved designing and validating a dd-PCR HHV assay, testing of whole blood samples and analytic analysis to evaluate the use of PCR as a diagnostic test in emergency departments. I aimed to determine how often HHV-6B was screened for, the rate of bacterial co-infection and evaluate the over-prescription of antimicrobials. My 3rd year dissertation project analysed the potential use of bacteriophages to be used as alternative therapies for diabetic foot ulcer infections. This project involved isolating novel phages and assessing their lytic capability against a range of clinical samples. This data was combined with sequencing data and bioinformatic analysis to determine the capabilities of potential phages.

I am now undertaking an MRes under the supervision of Dr Edward Emmott and my project is entitled ‘Regulation of norovirus infection by post-translational modification of viral proteins’. Utilizing mass spectrometry and cell culture, this project will investigate the composition and dynamics of norovirus replication complexes.


1

Join the team!

Like most labs, we have a decent number of applications for positions. A majority that are not competitive make common mistakes, the most important being it isn’t obvious why you want to work in ‘this’ lab. Ed has previously written a short twitter thread on applying to PhD’s and postdoc positions – take a look here.

 

Work in this lab will suit you if you:

  • Want to do rigorous science, where we share our data, code, and reagents, letting others learn from, expand on, and validate our results.
  • Find (+ssRNA) viruses neat! Both because they can cause a lot of damage, but also because they can be incredible tools to learn about normal cell biology.
  • Like mass spec (or want to learn), and how we can apply and develop new methods to study virus and cell biology.
  • Aren’t afraid of doing/learning some coding so you can do your own analysis (with help!).
  • Want to have fun, with a supportive team who help and troubleshoot each others work (and when necessary, feed each other cake) while you are doing it!

 

Funded Positions:

None current.  Please contact Ed if you wish to apply for projects with your own funding.

 

 

Unfunded Positions:

None current.  Please contact Ed if you wish to apply for projects with your own funding.

 

Please email Ed at e.emmott{at}liverpool.ac.uk if you have any questions about these positions.

 

Other opportunities:

Fellowships and applying with/for your own funding (PhD &. Postdoc): If you have access to, or wish to pursue alternate sources of funding for PhD or postdoctoral positions in the lab, please contact Ed with a copy of your CV, and a description of your research interests and how these can fit with research in the lab. If a good fit, Ed can work with you to identify suitable research questions, and assist with funding applications.

 

Details on the application process for international students with access to funding who wish to pursue PhD study can be found here. A great source of potential fellowship schemes for interested applicants can be found at ECRcentral. Some specific Postdoctoral/PhD funding schemes include:

 

Royal Society Newton International Fellowships (Postdoctoral)

International applicants may be eligible for Royal Society Newton International Fellowships which provide 2 years of funding for postdoctoral projects. Applicants must not be UK citizens, and must be working outside the UK at the time of application. Full eligibility notes available here.

 

Commenwealth Scholarships (PhD, Postdoctoral)

International applicants from Commonwealth countries may be eligible for Commonwealth Scholarships to support PhD or Postdoctoral studies. The exact funding schemes available depend on the applicant country of citizenship.

 

Summer Studentships: If you are a second or third year BSc student interested in a summer placement in the lab, please contact Ed early in the academic year. There are funding streams you can apply for, for example from the Microbiology Society , however the deadlines for these are generally early in the spring before the placement so please make contact early. It is usually Ed that will need to apply on your behalf, so you need to discuss this with Ed with plenty of time before the deadline.

 

Masters Projects: There are projects available in the lab on the MRes Advanced Biological Sciences course for 2021 start. Please refer to the course page for details of the course, and email Ed if you would like further details of the available projects.

 

Positions (General): All current positions (funded or unfunded) will be advertised here, on the lab twitter account (@emmottlab) and through University recruitment as they become available. PhD student positions will be advertised on findaphd.com.