Dr Ed (Edward) Emmott (PI)

Background: Ed joined the Centre for Proteome Research/Department of Biochemistry at the University of Liverpool to start the Emmott lab as a Tenure-Track Fellow in November 2019.

Research Interests: +ssRNA virus replication, virus-host interactions especially viral proteases & viral modification of ribonucleoprotein complexes (e.g. ribosomes), ultrasensitive/single-cell proteomic methods.

For more on Ed, click here.

Ed moved to the University of Liverpool in November 2019 to set up the lab  as part of the Centre for Proteome Research, Department of Biochemistry. The labs goal is the use and development of proteomic methods to study virus replication and virus-host interactions.

Previously, he was a Postdoc in Nikolai Slavov’s lab, part of the Department of Bioengineering and the Barnett Institute for Chemical and Biological Analysis at Northeastern University. His postdoctoral research with Nikolai involved the study of ribosome heterogeneity and the immune response, as well as the development and application of single cell proteomic methods (SCoPE-MS/SCoPE2).

Prior to joining Nikolai’s lab, Ed was a postdoc in Ian Goodfellow’s lab in the Department of Pathology at the University of Cambridge and Imperial College London where he studied norovirus replication and virus-host interactions, becoming particularly interested in the role of the viral protease, and polyprotein processing.

His PhD was at the University of Leeds  under the supervision of Julian Hiscox with his thesis on ‘High-throughput proteomic analysis of the interactions of avian coronavirus and its nucleocapsid with the host cell’. His Bachelors was from the University of Warwick in Medical Microbiology & Virology where his undergraduate research on Adenovirus interactions with PML bodies was supervised by Keith Leppard.

Twitter: @edemmott  ORCID: 0000-0002-3239-8178   CPR Page: (CPR)   Staff Page: (Liverpool)

Email: e.emmott{at}liverpool.ac.uk

 


1

Join the team!

Like most labs, we have a decent number of applications for positions. A majority that are not competitive make common mistakes, the most important being it isn’t obvious why you want to work in ‘this’ lab. Ed has previously written a short twitter thread on applying to PhD’s and postdoc positions – take a look here.

 

Work in this lab will suit you if you:

  • Want to do rigorous science, where we share our data and code, letting others learn from and validate our results.
  • Find (+ssRNA) viruses neat! Both because they can cause a lot of damage, but also because they can be incredible tools to learn about normal cell biology.
  • Like mass spec (or want to learn), and how we can apply and develop new methods to study virus and cell biology.
  • Aren’t afraid of doing/learning some coding so you can do your own analysis (with help!).
  • Want to have fun, with a supportive team who help and troubleshoot each others work (and when necessary, feed each other cake) while you are doing it!

 

Funded Positions:

None current.

 

Unfunded Positions:

PhD Studentship: Mass-spectrometry-based proteomic analysis of protease activity during SARS-CoV-2 infection. (Click for more details - Open advert - no deadline)

 

See this position on findaphd.com!

SARS-CoV-2 is the causative agent behind the COVID-19 pandemic, causing tens of millions of infections and hundreds of thousands of deaths globally. While licenced vaccines are likely to appear in the near future, there remain few therapeutic options available to treat COVID-19 disease.

You will build on preliminary and prior data from the Emmott lab on host protein cleavage during SARS-CoV-2 infection. We used mass spectrometry to identify 15 new cellular targets of the viral proteases, 12 of which resulted in a 100-fold drop in viral titres following siRNA inhibition, and several have inhibitors available in clinical use.  You will extend and combine this data with cutting-edge single-cell proteomic approaches to study how cleavage events vary between infected cells, and the impact this variation has on the outcome of coronavirus infection at single-cell level, and in vitro and cell-based assays to characterize and validate cleavage.

This project covers both ‘wet-lab’ and computational training. You will receive training in mass spectrometry including N-terminomics, single-cell proteomics (SCoPE2), in vitro and cell-based assays, data analysis in Matlab or R, including merging datasets, and standard cell culture and virological methods for handling SARS-CoV-2.

 

The project is suited to a student with at least a good B.Sc. Upper Second in Biological or Life Sciences (Especially in virology, biochemistry and systems biology/bioengineering).

If you are interested in applying please contact Dr Edward Emmott on 

 

The project is open to both European/UK and International students. It is UNFUNDED and applicants are encouraged to contact the Principal Supervisor directly to discuss their application and the project.

Assistance will be given to those who are applying to international funding schemes. The successful applicant will be expected to provide the funding for tuition fees and living expenses as well as research costs.

A fee bursary may be available for well qualified and motivated applicants. Details of costs can be found on the University website:

https://www.liverpool.ac.uk/study/postgraduate-research/fees-and-funding/fees-and-costs/

 

Subject areas:

Virology, Biochemistry, Cell Biology/Development, Bioinformatics, Immunology

 

Relevent Publications:

1. Meyer et al. (2020) Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing. bioRxiv, doi: 10.1101/665307
2. Emmott et al. (2019) Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity. J. Biol. Chem.294(11):4259-4271
3. Emmott et al. (2017) Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of initiation factors. Mol. Cell. Proteomics 16(4s1):S215-S229

See this position on findaphd.com!

 

Please email Ed at e.emmott{at}liverpool.ac.uk if you have any questions about these positions.

 

Other opportunities:

Fellowships and applying with/for your own funding (PhD &. Postdoc): If you have access to, or wish to pursue alternate sources of funding for PhD or postdoctoral positions in the lab, please contact Ed with a copy of your CV, and a description of your research interests and how these can fit with research in the lab. If a good fit, Ed can work with you to identify suitable research questions, and assist with funding applications.

 

Details on the application process for international students with access to funding who wish to pursue PhD study can be found here. A great source of potential fellowship schemes for interested applicants can be found at ECRcentral. Some specific Postdoctoral/PhD funding schemes include:

 

Royal Society Newton International Fellowships (Postdoctoral)

International applicants may be eligible for Royal Society Newton International Fellowships which provide 2 years of funding for postdoctoral projects. The scheme will reopen in Jan 2021. Applicants must not be UK citizens, and must be working outside the UK at the time of application. Full eligibility notes available here.

 

Commenwealth Scholarships (PhD, Postdoctoral)

International applicants from Commonwealth countries may be eligible for Commonwealth Scholarships to support PhD or Postdoctoral studies. The exact funding schemes available depend on the applicant country of citizenship.

 

Summer Studentships: If you are a second or third year BSc student interested in a summer placement in the lab, please contact Ed early in the academic year. There are funding streams you can apply for, for example from the Microbiology Society , however the deadlines for these are generally early in the spring before the placement so please make contact early. It is usually Ed that will need to apply on your behalf, so you need to discuss this with Ed with plenty of time before the deadline.

 

Masters Projects: There are projects available in the lab on the MRes Advanced Biological Sciences course for 2020 start. Please refer to the course page for details of the course, and email Ed if you would like further details of the available projects.

 

Positions (General): All current positions (funded or unfunded) will be advertised here, on the lab twitter account (@emmottlab) and through University recruitment as they become available. PhD student positions will be advertised on findaphd.com.